Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

关中平原城市群植被覆盖的时空特征与影响因素

植被是全球及区域生态系统环境变化的重要指标,也是对人类社会活动有重要贡献的资源之一。为了研究关中平原城市群不同区域植被覆盖变化对自然和人文因子的响应,以划分为三个区域的植被作为研究对象,选取2000—2017年MODIS-NDVI遥感数据,运用趋势分析、探索性空间数据分析与地理探测器等方法,从时序演进与空间分布方面研究了18 a内植被覆盖的演化及分布特征,定量分析影响植被覆盖的主导因子。遥感数据要通过投影转换、拼接、最大值合成等方法进行处理,再运用Python程序进行影像批量裁剪,将遥感数据和气象数据进行分区统计,最后对该处理数据进行讨论研究。结论表明：（1） 研究期内关中平原城市群植被覆盖呈显著上升趋势,NDVI平均值增速为0.077·（10 a）^–1,阶段性变化特征明显,其中2005—2007阶段和2011—2013阶段极显著增加,最大上升速率达到了0.05·a^–1。（2） 空间上总体呈现“南高北低”的分布特征,研究区总体得到改善;高值区主要分布在南部秦岭北坡,受气候因子的影响更大,植被覆盖增加速度缓慢,达到轻度改善水平;低值区聚集在黄土高原边缘地区,植被增加趋势明显;中部关中平原极少部分地区植被覆盖出现了轻度退化或严重退化,以西安市及临近城市最为典型。（3） 热点区主要分布在秦岭山区及关中平原中部地区,冷点区则集中于黄土高原边缘地区,植被覆盖总体以增长为主。热点区格网数量持续增多,2013年达到最大为45.07%;冷点区域数量不断减少,2017年减少到9.82%;次热点区与次冷点区主要分布在中部平原地带及北部地区,由连片分布转化为零散分布,且总量不断减少。（4） 自然因素对植被覆盖的影响最为突出,其中气温和降水为影响植被覆盖的主导因子,决定力q值分别为0.955和0.931,且气温的影响大于降水的影响;人文因子影响力较为显著,GDP因子决定力q值达到0.387。研究可为当地改善植被覆盖环境提供理论依据。     

A CASE STUDY ON CONCENTRATION AND DECENTRALIZATION：BEHAVIOR AND DYNAMIC MECHANISM OF SPATIAL EVOLUTION IN METROPOLITAN AREA，NANJING，CHINA

In rapid socio-economic development,the process of concentration and dispersal of various elements tends to be more dramatic,tremendously influencing the shaping and transformation of the space in metropolitan area.Survey of spatial concentration and decentralization has thus become a basic method in examining metropolitan spatial evolution.In this research,three elements were selected as the essential indicators of the process:demographic density distribu-tion,employment density distribution and business office location.Performance of these elements in Nanjing City was exam-ined historically.As Nanjing City could be regarded as a representative of metropolitan areas in China,its situation large-ly suggestes the general characteristics in similar areas of China.Hence based on the investigation of Nanjing City,four general implications were highlighted.First ,metropolitan areas in China are in a violent process and shift of spatial concentra-tion and decentralization.Second,from now to at least the near future,concentration will continue to be the central fea-ture.Third,the landscape of metropolitan areas basically exhibits a dual structure character.The gap in environmental and ecological qualities among different districts will continue for a long time.Fourth,Central Business District (CBD) is playing an important role in helping to convert the traditionally single-centered city structure into a polycentric one.     

青藏高原隆升的非线性动态有限元仿真研究

根据青藏高原的地质特征建立分析模型，采用3维动态有限元方法，在计算仿真板块速度场的基础上，计算在青藏高原的隆升过程中该地区地壳岩石的等效应力和位移随时间的变化，计算仿真得到的速度场与1998年GPS观测的速度场吻合良好；与过去一贯的假设相反，计算结果反映出地壳应力场不是静态的，而是此起彼伏，不断变化的，应力值最大且变化最剧烈的地区在克什米尔地区、鄂尔多斯地区和鲜水河－小江断裂带，与地震多发区域吻合。     

对青藏东北缘现今块体划分、运动及变形的初步研究

利用2维非连续变形分析方法（DDA），以位移代替围压作为边界约束力，研究青藏东北缘现今块体划分及其运动变形。根据该地区地质构造及地震活动，以GPS点测量位移作为模拟结果约束点，得出了较合理的块体划分模型和随时间演化的主应变分布图，并把应变高值区与近几年来发生的5级以上地震作对比，得出了研究区内地震危险性可能较大的区域。另外，对模拟的甘青块体与阿拉善块体的边缘带断裂左旋运动做了大概计算。     

Influence of the Choice of Core-Envelope Transition Point on the Binary Merger of Two Main-sequence Components

We have studied the influence of different choices of core-envelope transition point on the final merger of contact binaries with two main-sequence components. A binary of 1.00 + 0.90M⊙ with an initial orbital period of 0.35d is examined. The mass fraction of the primary mixed with the matter of the secondary, qmix, determined by the chosen core-envelope transition point, ranges from 0.04 to 1.00 in our analysis. If as qmix< 0.8, none of the     

Bacterial productivity in the Prydz Bay and its adjacent waters,Antarctic

1　IntroductionBacteriaandtheiractivitiesplayanimportantroleintheelementalbiogeochemicalcyclesandenergytransformingintheocean (Zhenetal.1 997) .DortchandPackard(1 989) proposedthatfoodwebsintheeutrophicwatersaredominatedbythebiomassofprimaryproducerswhilefoodwebsintheoligotrophicwatersaredominatedbythebiomassofmicrobes.Heterotrophicbacteriahadbeenshowntoplayanimportantroleinthedecompositionoflarge ,rapidlysinkingorganicparticleswithinandbelowtheeuphot iczone ,andfurthertoaffecttheelementaldyn…     

银盘恒星运动学和化学丰度与银河系结构

大样本的银盘恒星的运动学数据和元素丰度特征是研究银河系结构及演化，尤其是银盘形成和演化的2个重要探针。本文首先介绍了银河系的总体结构特征，然后较详细的综述了以上2个探针的重要性。对我国LAMOST项目在研究银河系结构方面，尤其是银盘的形成和演化方面的能力作了合理的分析。     

穆珠朗玛峰地区土壤中稀土元素的含量和分布

本文论述了珠穆朗玛峯地区土壤中稀土元素的含量,以及它们在土壤中的分布模式,并发现土壤中稀土元素的含量水平在很大程度上受土壤母质的制约。